Environmental Biodetection Products Incorporated

Specializing in Biomolecular Testing kits for Toxicity, Mutagenicity, and Genotoxicity.

For Rapid Detection of Genotoxicity and DNA Damage.

Product Line

EBPI Product Line

Product No: 5031

The SOS-ChromoTest kit is designed for Rapid Detection of Genotoxicity or DNA Damage.

The SOS-ChromoTest kit is based on a novel genetically engineered E.coli, which measures the primary response of a cell to genetic damage. In just a few hours, the kit provides a clear, completely objective measurement of the genotoxicity of a sample by a simple visual qualitative evaluation of the degree of DNA damage the cell experiences by observing the colour obtained. A more quantitative value may be obtained by spectrophotometry using a micro-plate reader. The SOS-Chromotest analyses are highly correlated with the Salmonella Ames test for certain materials. Te

Each kit contains all the reagents and disposable materials necessary to test at least four samples, including dilutions and controls.

A single SOS ChromoTest kit can be used for a total of 96 unit tests. These tests must include standards and controls (using at least 16 units). Thus, a single kit can screen 80 samples for yes/no responses.

For quantitative measurements, it is often necessary to allow for an adequate range of sample dilutions and replicates, thus testing five to ten samples with each kit. Below is an example plate used to detect the DNA damage when a genotoxic material is exposed to the SOS ChromoTest Bacteria.

Potential applications of the SOS-ChromoTest that take advantage of the genetically engineered strain of E. coli, including testing of effluents for possible mutagenic or carcinogenic genotoxic compounds. It provides for an evaluation of potential genotoxicity, and is a convenient research tool.

S9 Activation Enzymes S9 is a crude liver enzyme extract that can, under certain conditions, convert materials without any genotoxic activity to active genotoxic entities. The chemical process involved is probably different for different materials. In addition, the lifetime of the activated moieties is extremely variable: some may be extremely short-lived. This is the reason for incubating the S-9 with the bacteria and the tested material at the same time.

SOS-ChromoTest offers the following advantages over traditional methods:

Testing of pharmaceuticals for genotoxic activity.

Testing of industrial effluents for presence of possible genotoxic compounds.

Screening of municipal discharges for possible routine presence or spills of genotoxic compounds.

Screening of surface and ground water for genotoxic residues.

Screening of potable water supplies for the presence of chemicals with genotoxic potential.

Screening of water soluble air pollutants for genotoxic agents.

Evaluation of pure or complexed raw mixtures for potential genotoxicity.

A convenient and easy to use teaching look for university and college laboratories.

The SOS-ChromoTest kit is designed to be user friendly, reliable, sensitive, rapid, low cost and contains all plastics required to complete the assay.

Click Here for a visual understanding on how the SOS-ChromoTest kit is preformed

2010 Pricing